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                                                陳佳    助理教授、研究員
所在學院生命科學與技術學院
研究方向DNA修復、基因編輯與癌癥發生
聯系方式chenjia@@shanghaitech.edu.cn
備注優青


個人簡介
2002年本科畢業于南開大學生命科學學院生物化學系百万彩票注册链接;
2009年在中科院上海生物化學與細胞生物學研究所獲博士學位;
2009年至2014年在美國國立衛生研究院糖尿病、消化道和腎臟疾病研究所(NIH/NIDDK)進行博士后研究工作。
2014年11月加入上喊偻虿势弊⒉崃唇樱科技大學生命科學與技術學院任助理教授、研究員。
2016年獲得上海市科委浦江人才計劃資助,2018年獲得國家自然科學基金委優秀青年科學基金資助。
目前承擔國家自然科學基金委青年項目、面上項目、優青項目,科技部重點研發計劃等項目百万彩票注册链接。

主要研究內容
物種繁衍與基因組的穩定性密切相關百万彩票注册链接,這在細胞內是由一系列精巧而復雜的DNA修復機制負責維持的百万彩票注册链接。然而在某些生理、病理狀態下細胞會激活錯誤傾向性DNA修復機制,因此DNA修復精確性和錯誤傾向性之間的平衡對于疾病發生、衰老和進化都具有重要的意義。

基因編輯指利用可設計的核酸酶通過堿基插入、缺失、置換等方式百万彩票注册链接,對生物體基因組DNA特定片段進行改造從而達到對目標基因進行編輯的一種基因工程百万彩票注册链接,可廣泛應用于生命科學基礎研究、生物技術開發、農業技術開發以及醫藥研發領域。傳統的CRISPR/Cas基因編輯技術雖然具有較高的基因敲除效率,但在執行堿基替換時效率通常較低。近年內,將CRISPR/Cas與核酸脫氨酶整合發展出的堿基編輯系統百万彩票注册链接,可在單堿基水平對基因組實現高效率的靶向性編輯改造。

我們實驗室長期從事DNA修復以及基因編輯相關的研究工作,已闡明胞嘧啶脫氨酶APOBEC在CRISPR/Cas9介導的基因編輯過程中產生突變的分子機制,成功創建更高精度和更高效率的增強型Cas9堿基編輯器(eBE)、可在基因組A/T富集區域內開展有效編輯的Cpf1堿基編輯器(dCpf1-BE)、以及可在G/C富集區域和高甲基化區域內開展高效編輯的普適型Cas9堿基編輯器(hA3A-BE)。

未來幾年中百万彩票注册链接,我們實驗室將進一步探索由DNA修復引發的突變在基因編輯、疾病發生以及衰老等過程中所起的作用, 主要集中于以下幾個方面: 1)研究DNA修復在基因編輯過程中引發突變的分子機制;2)創建新型基因編輯系統;3)鑒定并分析DNA修復引發突變的新分子和新通路百万彩票注册链接;4)探索DNA修復引發突變在癌癥發生以及衰老過程中的作用百万彩票注册链接。我們的研究將有助于發展新型基因編輯系統,揭示癌癥發生的新機制以及完善衰老的DNA損傷學說百万彩票注册链接。

代表性論文

(# co-first author, * corresponding author)


1.Jia Chen*, Bei Yang* and Li Yang*. To BE or not to BE, that is the question. Nat Biotechnol, 2019, doi: 10.1038/s41587-019-0119-x


2.Bei Yang*, Li Yang* and Jia Chen*. Development and Application of Base Editors. CRISPR J, 2019, 2: 91-104


3.Jianan Li#, Zhen Liu#, Shisheng Huang#, Xiao Wang, Guanglei Li, Yuting Xu, Wenxia Yu, Shanshan Chen, Yu Zhang, Hanhui Ma, Zunfu Ke, Jia Chen*, Qiang Sun* and Xingxu Huang*. Efficient base editing in G/C-rich regions to model androgen insensitivity syndrome. Cell Res, 2019, 29: 174-176


4.Xiao Wang#, Jianan Li#, Ying Wang#, Bei Yang#, Jia Wei#, Jing Wu, Ruixuan Wang, Xingxu Huang*, Jia Chen* and Li Yang*. Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol, 2018, 36: 946-949 (Highlighted by Nicole Rusk, Better base editors. Nat Methods, 2018, 15:763)


5.Yanting Zeng#, Jianan Li#, Guanglei Li#, Shisheng Huang, Wenxia Yu, Yu Zhang, Dunjin Chen, Jia Chen, Jianqiao Liu* and Xingxu Huang*. Correction of the Marfan Syndrome pathogenic FBN1 mutation by base editing in human cells and heterozygous embryos. Mol Ther, 2018, 26: 2631-2637


6.Zhen Liu#, Zongyang Lu#, Guang Yang#, Shisheng Huang, Guanglei Li, Songjie Feng, Yajing Liu, Jianan Li, Wenxia Yu, Yu Zhang, Jia Chen, Qiang Sun* and Xingxu Huang*. Efficient generation of mouse models of human diseases via ABE-and BE-mediated base editing. Nat Commun, 2018, 9: 2338


7.Wen Jiang#, Songjie Feng#, Shisheng Huang, Wenxia Yu, Guanglei Li, Guang Yang, Yajing Liu, Yu Zhang, Lei Zhang, Yu Hou, Jia Chen, Jieping Chen* and Xingxu Huang*. BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity. Cell Res, 2018, 28: 855-861


8.Jia Chen, Weizhi Ji, Prashant Mali and April Pawluk. The Future of Genome Editing. Cell, 2018, 173: 1311-1313


9.Xiaosa Li#, Ying Wang#, Yajing Liu#, Bei Yang#, Xiao Wang, Jia Wei, Zongyang Lu, Yuxi Zhang, Jing Wu, Xingxu Huang*, Li Yang* and Jia Chen*. Base editing with a cpf1-cytidine deaminase fusion. Nat Biotechnol, 2018, 36: 324-327 (Highlighted in Tools in Brief, Expanding the range of base editors. Nat Methods, 2018, 15:314)


10.Liqun Lei#, Hongquan Chen#, Wei Xue#, Bei Yang#, Bian Hu#, Jia Wei, Lijie Wang, Yiqiang Cui, Wei Li, Jianying Wang, Lei Yan, Wanjing Shang, Jimin Gao, Jiahao Sha, Min Zhuang, Xingxu Huang, Bin Shen*, Li Yang* and Jia Chen*. APOBEC3 induces mutations during repair of CRISPR–Cas9-generated DNA breaks. Nat Struct Mol Biol, 2018, 25: 45-52


11.Lijie Wang#, Wei Xue#, Lei Yan#, Xiaosa Li, Jia Wei, Miaomiao Chen, Jing Wu, Bei Yang*, Li Yang* and Jia Chen*. Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Cell Res, 2017, 27: 1289-1292


12.Guanglei Li, Yajing Liu, Yanting Zeng, Jianan Li, Lijie Wang, Guang Yang, Dunjin Chen, Xiaoyun Shang, Jia Chen, Xingxu Huang* and Jianqiao Liu*. Highly efficient and precise base editing in discarded human tripronuclear embryos. Protein Cell, 2017, 8: 776-779


13.Bei Yang*Xiaosa Li, Liqun Lei and Jia Chen*. APOBEC: from mutator to editor. J Genet Genomics2017, 44: 423-437


14.Jia Chenand Anthony V. Furano*. Breaking bad: The mutagenic effect of DNA repair. DNA Repair2015, 32: 43-51


15.Jia Chen, Brendan F. Miller and Anthony V. Furano*. Repair of naturally occurring mismatches can induce mutations in flanking DNA.eLife, 2014, 3: e02001 (Highlighted by Samuel H. Wilson, The dark side of DNA repair. eLife, 2014, 3:e03068)


16.Guang-Jing Hu#, Jia Chen#, Xiao-Nan Zhao#, Jia-Jia Xu, Dong-Qing Guo, Ming Lu, Ming Zhu, Ying Xiong, Qin Li, Catherine CY Chang, Bao-Liang Song, Ta-Yuan Chang and Bo-Liang Li*. Production of ACAT1 56-kDa isoform in human cells via trans-splicing involving the ampicillin resistance gene. Cell Res, 2013, 23: 1007-1024 (Cover storyHighlighted by Christian Preu?er and Albrecht Bindereif, Exo-endo trans splicing: a new way to link. Cell Res, 2013, 23: 1071-1072)


17.Lei Lei, Ying Xiong, Jia Chen, Jin-Bo Yang, Yi Wang, Xin-Ying Yang, Cantherine C. Y. Chang, Bao-Liang Song, Ta-Yuan Chang and Bo-Liang Li*. TNF-alpha stimulates the ACAT1 expression in differentiating monocytes to promote the CE-laden cell formation. J Lipid Res, 2009, 50: 1057-1067


18.Xiao-Nan Zhao#,Jia Chen#, Lei Lei, Guang-Jing Hu, Ying Xiong, Jia-Jia Xu, Qin Li, Xin-Ying Yang, Catherine CY Chang, Bao-Liang Song, Ta-Yuan Chang and Bo-Liang Li*. The optional long 5'-untranslated region of human ACAT1 mRNAs impairs the production of ACAT1 protein by promoting its mRNA decay. Acta Biochim Biophys Sin, 2009, 41: 30-41


19.Jia Chen#, Xiao-Nan Zhao#, Li Yang, Guang-Jing Hu, Ming Lu, Ying Xiong, Xin-Ying Yang, Catherine CY Chang, Bao-Liang Song, Ta-Yuan Chang and Bo-Liang Li*. RNA secondary structures located in the interchromosomal region of human ACAT1 chimeric mRNA are required to produce the 56-kDa isoform. Cell Res, 2008, 18: 921-936


20.Bo-Liang Li*, Ta-Yuan Chang, Jia Chen, Catherine CY Chang and Xiao-Nan Zhao. Human ACAT1 gene expression and its involvement in the development of atherosclerosis. Future Cardiol, 2006, 2: 93-99


21.Li Yang, Oneil Lee, Jia Chen, Jiang Chen, Catherine C.Y. Chang, Pei Zhou, Zhen-Zhen Wang, Han-Hui Ma, Hui-Fang Sha, Jiu-Xian Feng, Yi Wang, Xin-Ying Yang, Li Wang, Ruhong Dong, Kim Ornvold, Bo-Liang Li* and Ta-Yuan Chang*. Human acyl-coenzyme A:cholesterol acyltransferase 1(Acat1) sequences located in two different chromosomes (7 and 1) are required to produce a novel ACAT1 isoenzyme with additional sequence at the N-terminal. J Biol Chem, 2004, 279: 46253-46262


22.Li Yang, Jin-Bo Yang, Jia Chen, Guang-Yao Yu, Pei Zhou, Lei Lei, Zhen-Zhen Wang, Catherine C.Y. Chang, Xin-Ying Yang, Ta-Yuan Chang* and Bo-Liang Li*. Enhancement of human ACAT1 gene expression to promote the macrophage-derived foam cell formation by dexamethasone. Cell Res, 2004, 14: 315-323 (Cover story)



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